Structural characterization of a recombinant fusion protein by instrumental analysis and molecular modeling.

Structural characterization of a recombinant fusion protein by instrumental analysis and molecular modeling.

Blog Article

Conbercept Motorcycle Kit is a genetically engineered homodimeric protein for the treatment of wet age-related macular degeneration (wet AMD) that functions by blocking VEGF-family proteins.Its huge, highly variable architecture makes characterization and development of a functional assay difficult.In this study, the primary structure, number of disulfide linkages and glycosylation state of conbercept were characterized by high-performance liquid chromatography, mass spectrometry, and capillary electrophoresis.Molecular modeling was then applied to obtain the spatial structural model of the conbercept-VEGF-A Rolling Papers complex, and to study its inter-atomic interactions and dynamic behavior.This work was incorporated into a platform useful for studying the structure of conbercept and its ligand binding functions.